For example, replacing septa or liner at regular intervals and keeping the injector and detector clean and wellmaintained shoud solve many problems. Detecting lowabundance compounds in complex matrices, that continually change, is a challenge. Other people in the lab may have had experience solving a problem that is giving you trouble. In cases like this, the flowchart approach on the last slide will help to isolate the source of the problem. Truncating at 99 search is returning too many results solution. Compare the latest chromatogram with an earlier one, if existing, but compare the two results graphically. Clarus 500 gc hardware guide 15 conventions used in this manual normal text is used to provide information and instructions. Gc columns and accessories gc technical information symptom cause recommended solutions peakrelated problems peaks broadening column fl ow too high. This gas chromatography and troubleshooting for the oil and gas industry training course commences with an introduction to gas chromatography and will cover the following areas.
The problem is not new, it has happened sporadically since the instrument and software were installed about a year ago, but has now become more and more frequent. View and download shimadzu gc2014 instruction manual online. A logical approach to gc troubleshooting and maintenance is explored in this oneday course. This product is manufactured by new england biolabs, inc. Pcr troubleshooting part 1 no bands by matt bernstein technical support while the days of mineral oil and 2minute ramp times are almost entirely a thing of the past, failed pcr is still as much a presence as it ever was. The topics are grouped into cylinder safety, general. For the problem identification we recommend to use electronic flowmeter and leak detector. Gc troubleshooting and maintenance crawford scientific. Page 1 page 2 page 3 page 4 page 5 table of contents page 6 page 7 page 8 page 9 page 10 page 11 page 12 page installation page 14 page 15 page 16 power supply and wiring page 17 page 18 page 19 gas supply plumbing page 20 page 21 page 22 page 23 page 24 page 25 page 26 page 27 page 28 page 29 before use page 30 page 31 page 32. Know the chemical and physical properties of any solvents used and have the appropriate material safety data sheets msdss readily.
Warning always configure the gc appropriately when working with hydrogen. Quick start guide chromeleon 7 thermo fisher scientific. Gc troubleshooting is a very broad topic, and we could easily devote an entire day to talking about it. Page 36 condensation test procedure leave gc at 4050c for 8 hours blank run. Chemstation stops working unexpectedly agilent community. Gc troubleshooting guide phenomenex uhplc, hplc, spe and gc. This gc unit has particularly distinguishing features. Be aware, however, that overtightened metal compression fittings can leak and plasrtic fingertight fittings can wear out. Agilent 7697a troubleshooting 11 keeping configuration settings current certain configurable items in the hs must always be kept current. Gas chromatography and troubleshooting training course. Application log files and analyzing errors in log files.
Logic something changed slowly or sudden something is different track events log bookchanged column, liner, septum, syringe, etc. Reconfigure the gc every time the gas type is changed. If the action is a question a question will have a number in the. I provide this information to help educate the readers. Client is not gc owner as required to perform command. Edited for clarityformat, added parts from user guide 2. Preventing problems the best way to solve problems is to prevent them.
Agilent 5973 gcms training manual iowa state university. Poor column installation causing dead volume in the. General tasks concepts this manual provides lists of symptoms and corresponding tasks to perform should you experience errors associated with gc hardware or chromatographic output, gc not ready messages, and other common. Baseline problems could be divided into 5 categories. If the flow rates are not correct the fid can give an incorrect response for a sample. All eight digit numbers are perkinelmer part numbers unless stated otherwise.
Close all open programs before beginning the disk check. Use our tm calculator to help plan experiments and click here for optimization tips. Temperature control errors 1009 aux5 temp exceed the limit these messages appear when the maximum temperature limit has been exceeded. Ftir troubleshooting guide using our vast ftir service experience we have compiled this list of symptoms and causes for ailments common to all ftir systems, regardless of the vendor. Pdf analytical errors in routine gas chromatography analysis.
Do whatever the action says, then proceed to the step indicated in the go to column. Phusion dna polymerase was developed by finnzymes oy, now a part of thermo fisher scientific. T he main tests to help detecting and reducing systematic quantitative gc errors 1. Page 6 typical problems of optimized methods becoming unoptimizedand the reason why. Hplc troubleshooting hints and tips chromatography. Gas chromatograph software interface user manual for fb107 ii revised june2017 revision tracking sheet june 2017 this manual may be revised periodically to incorporate new or updated information. If you are performing multiple collector steps, using the clear collected action may help reduce the load. In the past 50 years, gas chromatography has become an important analytical tool in virtually every phase of the petroleum industry, from exploration of crude oil and refining of finished products to research on new petrochemicals. Regular preventive maintenance depends on particular model of gc and you should consult required operations in the operators and service manuals.
The sample solution injected into the instrument enters a gas stream which transports the sample into. Frequently asked questions about chromatographic gases. These logs may contain valuable information that directly indicates the root cause of problem. A poorly installed column or very slow injection speed can cause peak tailing. Jan 21, 2020 sap netweaver application server java. Diagnosing and resolving mass spec problems april 2017 note. Bad mainboard and fatal errors these messages almost always indicate that the mainboard is malfunctioning and must be replaced. Gc troubleshooting gc troubleshooting hints and tips. Perkinelmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing, performance or use of this material. A practical users guide, second edition published online. Usually, the earlier eluting peaks exhibit the worst tailing for these types of problems.
Commonly encountered problems and best practices are examined for all major system components including gas handling, inlet system, oven, column and detector. Agilent 7697a headspace sampler troubleshooting guide. Sample loop size reconfigure the sample loop size whenever you change the sample loop. Error in sample concentration verify concentrations. The complete guide to gc troubleshooting with hints, tips, and tricks for chromatographic remedies.
Clarus 500580 gc users guide 17 glossary of clarus gc terms the glossary of clarus gc terms are divided into two types. Gc troubleshooting the technique of gc is inherently complex with many pieces of equipment interacting to analyse a sample and to produce a chromatogram as a result. A surprising number of problems involve fairly simple and often overlooked. Have your instrument manual and these diagnostic tools at hand. This guide also covers basic information for troubleshooting your gc systems. You can meet that challenge and exceed regulatory demands for known, new and emerging compounds in every run with sciex mass spec solutions. And even though the technology out there now is greater than ever, with more labs doing. I realize that its a full time job just to run a gcmswhat with. A thermal runaway condition exists for the gc oven. Agilent technologies 6890n troubleshooting manual pdf download. The analysis performed by a gas chromatograph is called gas chromatography.
Leaks are usually stopped by tightening or replacing a fitting. The following guide can be used to troubleshoot pcr reactions. Check sample handling steps for potential contamination. Troubleshooting techniques, tips, and tricks daron decker chromatography technical specialist page 2 everything was just fine and then this happened. These messages are not numbered and usually appear. Thank you very much for your purchase of shimadzus gc14b series gas chromatograph. Hydrogen leaks quickly and poses a safety concern if too much of it is released into the air or into the gc oven.
Many gc problems can be prevented if the column and system are maintained routinely. It is also a supplement to the perkinelmer standard safety and. The gc14b series gas chromatograph, which is controlled by microcomputer, is a multipurpose gas chromatograph exhibiting high performance. Most gc manufacturers offer free technical support to their. Install and maintain proper purification for all gases in the gc system. Troubleshooting gas chromatograph baseline problems. Repurify, or precipitate and wash dna with 70% ethanol, to remove residual salts or ions e. If youre new to gas chromatography and looking for some helpful tips, here are 8 common mistakes youll want to avoid. Modifications and updates version date description of change 1. A gas chromatograph gc is an analytical instrument that measures the content of various components in a sample. This advice is intended to supplement, not supersede, the normal safety codes in the users country.
Many gc problems can be prevented if the column is properly installed and gc is. Here is a list of support options and recommendations, including frequently asked questions faqs, to help you achieve your goals. Troubleshooting strategy approaching the problem check first to see if a fix for the problem is already known. Each section describes a problem and provides a bulleted list of. If a fitting leak does not stop when the fitting is tightened a little, take the fitting apart and inspect for damage e.
If the gc is configured to a gas other than what is actually being plumbed, incorrect flow rates will result. Use when problems are worse after periods of gc nonuse. The gc 14b series gas chromatograph, which is controlled by microcomputer, is a multipurpose gas chromatograph exhibiting high performance. Electrical connections signal connections gas purity. Frequently asked questions about chromatographic gases a. The revision date of each page appears at the bottom of the page.
Troubleshooting and maintenance guide hewlett packard. In such a system the potential for things to go wrong is necessarily high if operators do not know what they are doing or are less than careful in their approach to. Hi, we have had a recurring issue with our chemstation software connected to our agilent 1260 infinity ii systems. The sample solution injected into the instrument enters a gas stream which transports the sample into a separation tube. Many gc problems can be prevented if the column is properly installed and gc is maintained routinely. Troubleshooting gas chromatograph baseline problems a16089 this document is concerned with diagnosis. Gc troubleshooting and maintenance a logical approach to gc troubleshooting and maintenance is explored in this oneday course.
Failure to do so will lead to reduced sensitivity, chromatographic errors, and possible safety concerns. This publication is in a questionandanswer format, something like the faq frequently asked questions. The empower gas chromatography getting started guide describes the basics of how to set up gc instruments with your empower system, how to develop a gc instrument method, and how to use custom fields to enhance your gc data. Line chromatograms can be checked quickly, quantitatively, completely.
Pcr troubleshooting guide thermo fisher scientific us. Gc columns and accessories thermo scientifi c chromatography columns and consumables 20142015 3160 gc troubleshooting before you start any troubleshooting, it is essential to observe safe laboratory practices. Useful information and hints for troubleshooting problems. Follow these basic troubleshooting steps to isolate problems related to the sample, injector, detector, and column.
Basic capillary gc theory and practical troubleshooting. Thank you very much for your purchase of shimadzus gc 14b series gas chromatograph. Terminal typical wire color function y yellow cool w white heat g green fan r red 24vac return c. Clarus 500 gc hardware guide 23 chapter overview this chapter describes the general safety practices and precautions that must be observed when operating the clarus 500 gc. In this webinar, i will be focusing on helping you to develop general troubleshooting strategies and give you some things to consider when things do not go as planned in a gc analysis. Gcms troubleshooting quick reference wiley online library. Some problems, such as peak shape, can originate from multiple sources. Bold text refers to button or tab page that is displayed on the touch screen. View and download agilent technologies 6890n troubleshooting manual online. Chemstation pdf agilent guide to using chemstation 5973 msd guide pdf this cif guide for using the 5973 msd, chemstation, and masshunter snagit 10 starts screencapture. Each section describes a problem and provides a bulleted list. Hello everyone, and thank you for attending this webinar.